do11 10 transgenic mice  (Jackson Laboratory)

Journal: PLoS ONE

Article Title: Optimal Population of FoxP3 + T Cells in Tumors Requires an Antigen Priming-Dependent Trafficking Receptor Switch

doi: 10.1371/journal.pone.0030793

Figure Lengend Snippet: (A) Splenocytes of DO11.10 Rag2−/− mice (containing naïve FoxP3 − CD4 + T cells but not FoxP3 + T cells) were injected i.v. into BALB/c mice, and A20 tumor cells expressing OVA were implanted 15 hours later. Mice were sacrificed 2 weeks later, and the conversion rate of naïve FoxP3 − CD4 + T cells into FoxP3 + T cells (represented by % FoxP3 + of KJ-1.26 + CD4 + T cells) was determined in various organs and tumors. (B) Memory FoxP3 + T cells are enriched in tumors. Expression of CD44 and CD62L by FoxP3 + T cells in tumors and peripheral lymph nodes were compared. Representative and combined data obtained from 3 (A) or 5–8 different experiments (B) are shown. “*” indicates significant differences between the tumor and indicated organs (A) or PLN (B).

Article Snippet: DO11.10 TCR Rag2 (−/−) transgenic mice were purchased from Taconic Farms.

Techniques: Injection, Expressing

Journal: PLoS ONE

Article Title: Optimal Population of FoxP3 + T Cells in Tumors Requires an Antigen Priming-Dependent Trafficking Receptor Switch

doi: 10.1371/journal.pone.0030793

Figure Lengend Snippet: (A) Loss of CCR7 on FoxP3 + T cells following antigen priming. OVA-specific FoxP3 + T cells isolated from RIP-mOVA×DO11.10 Rag2 (−/−)mice were cultured in the presence of the OVA 323–339 peptide and irradiated splenocytes as antigen presenting cells for 7 days, and CCR7 expression by FoxP3 + T cells was examined. (B) Comparison of the homing ability of antigen primed versus naïve FoxP3 + T cells. Fresh and antigen-primed FoxP3 + T cells were co-injected i.v. into 4T1-OVA tumor-bearing mice and the relative migration of the two FoxP3 + T cell populations into tumors and other organs were determined 36 h following the injection. (C) Comparison of antigen primed wild type and CCR7 (−/−) FoxP3 + T cells into tumors. CD4 + T cells, isolated from wild type and CCR7−/− mice were antigen primed in vitro for 5 days and injected into B16-tumor bearing mice. The relative migration of the two FoxP3 + T cell populations into tumors and other organs were determined 36 h following the injection. Representative and combined data obtained from 4–5 different experiments are shown. “*” indicates significant differences between the tumor and indicated organs (B) or between WT and CCR7 (−/−) FoxP3 + T cells (C).

Article Snippet: DO11.10 TCR Rag2 (−/−) transgenic mice were purchased from Taconic Farms.

Techniques: Isolation, Cell Culture, Irradiation, Expressing, Comparison, Injection, Migration, In Vitro